Fusion Protein Expression Systems

Steve smiles at bgsm.edu
Fri Feb 14 14:24:38 EST 1997


I'm working on trying to overexpress a membrane protein in a fusion 
system. I've tried the maltose binding protein fusions and Intein 
fusions as well as Histidine tags. They haven't been very successful in 
overexpressing the protein. However I do get some reactivity on a 
western, show that there is some of my target protein made. Are there any 
other systems you guys and gals have tried that work pretty good? Just 

Take care. 

More information about the Methods mailing list