Fusion Protein Expression Systems

Steve smiles at bgsm.edu
Fri Feb 14 14:24:38 EST 1997


Hi!

I'm working on trying to overexpress a membrane protein in a fusion 
system. I've tried the maltose binding protein fusions and Intein 
fusions as well as Histidine tags. They haven't been very successful in 
overexpressing the protein. However I do get some reactivity on a 
western, show that there is some of my target protein made. Are there any 
other systems you guys and gals have tried that work pretty good? Just 
wondering.

Take care. 
Steve



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