Real time PCR measurement

Dr. J.P. Clewley jclewley at hgmp.mrc.ac.uk
Fri Feb 14 12:46:10 EST 1997


As far as I know there are 3 systems for real time measurement of a PCR:

TaqMan from PE Applied Biosystems

AmpliSensor from Biotronics

LightCycler from Idaho Technology

Anyone have any comments about the relative merits of these systems?
Reliability? Accuracy for quantitation? Throughput? Cost? Sensitivity?
Specificity?

I'll summarise answers.

Jon Clewley
Virus Reference Division
Central Public Health Laboratory
London, UK

Email: jclewley at hgmp.mrc.ac.uk



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