Disposal of Hoechst waste

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Sat Feb 15 11:16:06 EST 1997

For a MSDS, you might have a look at 

(Acros chmicals, no affil).

In fact, there's not much information about that stuff.

I would suggest to put it to the organic waste to be incinerated. 
Since there are now some colum-devices to extract ethidium bromide 
from solns, they might be effective for BBI, too. But I haven't tried 
this yet. If you have one handy, it might be easy to prove it.
Passing the soln after neutralizing through a strong anionic resin 
(-SO3) should remove the dye, too. 

My 2 cents


> In article
> <Pine.SOL.3.95q.970212115159.19406A-100000 at suma3.reading.ac.uk>, "J.
> G. Gibbings" <sbsgibin at reading.ac.uk> wrote:
> > Hi,
> >         Can anyone out there advise me on how to dispose of the
> >         possible
> > mutagenic Hoechst dye waste solutions (0.1mg/L). We use the dye to
> > quantitate DNA on the DyNA Quant 200 Hoefer flurometer. 
> >         Thanks.
> > 
> >         George.
> George:
> If you are very conscientious you could bind the dye to activated
> charcoal and have that incinerated, but if you're dealing with litre
> quantities of solution that procedure would be pretty laborious. 
> Probably the one thing that everybody would agree on is that you
> shouldn't treat the dye with hypochlorite bleach, because although
> that will decolourize it, it will almost certainly convert the dye
> into various genuinely undesirable chemicals.
> I am not aware of any evidence that the Hoechst dyes (bisbenzimides)
> are in fact mutagenic.  As was demonstrated by some nice work by
> Werner Mueller in the mid 70's (he has three papers in Eur. J.
> Biochem. 54, 1975; for this specific dye see Eur. J. Biochem. 54,
> 385-394 (1975) ) Hoechst/bisbenzimide is not an intercalating dye
> as, for example, ethidium bromide or the acridines are.  In fact he
> determined that apparently all dyes that have A-T binding
> preferences (this would include DAPI, Berenil, methyl green and the
> bisbenzimides, although he studied only the latter two of this
> group) bind to the outside of the DNA helix. Intercalating dyes by
> contrast have slight to high preferences for G-C rich regions in DNA
> and they obviously have the potential for generating frame shift
> mutations if they are bound to replicating DNA; some members of this
> group have photosensitizing or photodynamic activities which can in
> appropriate circumstances cause DNA damage.
> I would suggest that Hoechst dyes represent no greater threat
> (possibly less) than say Coomassie Blue and any number of standard
> lab dyes that have never been implicated in anything worse than
> possibly mild skin irritation. (The whole issue of ethidium bromide
> disposal is of course frequently engulfed in an irrational
> hysteria.)
> The bisbenzimides were originally screened (if I recall correctly)
> as potential trypanocide or maybe antimalarial drugs, so at worst
> the dye might present an environmental threat to Trypanosoma or
> Plasmodium species;  neither group seems to have many apologists.
> Dave Spencer
> -- 
> David F. Spencer, PhD
> Dept. of Biochemistry
> Dalhousie University
> Halifax, Nova Scotia, Canada
> dspencer at is.dal.ca
> dspencer at rsu.biochem.dal.ca
---------!all possible disclaimers apply!-----------
Wolfgang Schechinger
University of Tuebingen
email: wgschech at med.uni-tuebingen.de


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