IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

large amount of mRNA is needed

hendrikb hendrikb at worldonline.nl
Sat Feb 15 08:05:58 EST 1997

Wei Shao <shaow at FCRFV1.NCIFCRF.GOV> wrote in artikel
<32F19DDF.2E2A at fcrfv1.ncifcrf.gov>...
> Hello all, is there any vector good for making large amount of mRNA 
> from a cloned gene? I do not need expressed protein, only need 
> mRNA. Promega has some trancription vectors. I think those are for 
> isolating mRNA for in vitro transcription. What I need is isolating 
> mRNA of cloned gene from bacterial in mg level-- Is it possible?
> Thanks.
> Wei
I don't know a method to get such large amount. We used  the message
machine kit of Ambion to get really large amounts of clean mRNA. Good luck.
Brenda Hendriks

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net