Wei Shao <shaow at FCRFV1.NCIFCRF.GOV> wrote in artikel
<32F19DDF.2E2A at fcrfv1.ncifcrf.gov>...
> Hello all, is there any vector good for making large amount of mRNA
> from a cloned gene? I do not need expressed protein, only need
> mRNA. Promega has some trancription vectors. I think those are for
> isolating mRNA for in vitro transcription. What I need is isolating
> mRNA of cloned gene from bacterial in mg level-- Is it possible?
>I don't know a method to get such large amount. We used the message
machine kit of Ambion to get really large amounts of clean mRNA. Good luck.