large amount of mRNA is needed

hendrikb hendrikb at worldonline.nl
Sat Feb 15 08:05:58 EST 1997



Wei Shao <shaow at FCRFV1.NCIFCRF.GOV> wrote in artikel
<32F19DDF.2E2A at fcrfv1.ncifcrf.gov>...
> Hello all, is there any vector good for making large amount of mRNA 
> from a cloned gene? I do not need expressed protein, only need 
> mRNA. Promega has some trancription vectors. I think those are for 
> isolating mRNA for in vitro transcription. What I need is isolating 
> mRNA of cloned gene from bacterial in mg level-- Is it possible?
> Thanks.
> Wei
> 
I don't know a method to get such large amount. We used  the message
machine kit of Ambion to get really large amounts of clean mRNA. Good luck.
Brenda Hendriks



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