Matthew David Petroski
MDPETROS at UCI.EDU
Sat Feb 15 14:41:48 EST 1997
I am looking for a suggestion as to how to remove SDS from protein samples
containing sub-microgram amounts of a highly purified protein. I am
electroeluting protein samples from native polyacrylamide gels. I need to
concentrate the samples from about 1 ml down to about 20 ul. I would rather not
precipitate this as the samples are quite dilute and the amount of loss is
extremely high. Also, I cannot omit SDS from the electroelution buffer as my
protein does not seem to elute well without it. I would like to somehow reduce
the amount of SDS and subsequently lyophilize.
Would dialysis be effective? I know that SDS forms micelles which I think would
be extremely difficult (and inefficient) to dialyze, but I have seen a
publication which claims to have done this...
I have also seen detergent removal columns from several companies. The problem
with these, however, seems to be that they all seem to require concentrations of
greater than ug/ml or "severe losses will occur".
Suggestions will be greatly appreciated!
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