"Oligonucleotide purification method??"

Paul Dunman dunman at njmsa.UMDNJ.EDU
Sat Feb 15 14:15:55 EST 1997


xie at assel.biochem.mpg.de (Youhua Xie) writes:

>Balwi (M.Albalwi at sheffield.ac.uk) wrote:
>: I have labelled some oligonucleotide with dig-11-dutp at 3'end, but I 
>: did
>: not know which best method to clean unincorporated nucleotide
>: (dig-11-dutp). Is it by using preceiptation method(Na-acetat/ethanol) 
>: or by
>: using spun colum. If you think you have idea please e-mail me.
>I found Qiagen's nucleotide remove kit quite useful for the separation.

>xie

I believe Na-Acetate will also ppt. out the free nucleotides, try 
Ammonium Acetate.

Paul D.





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