Huang Ke-xue (khuang at CHEMVX.CHEM.TAMU.EDU) wrote: : Hi, Netters, : I met a serious problem recently. The transformed BL21(DE3)(by a expression : plasmid) lyses automatically after 6-8 hr growing. I repeated this three times. : Do you think my stuff was contamined by Phage(?). : Any suggestion? Try another DE3 lysogen?