E. coli (Bl21DE3) lyses automatically

Greg jquinn at nntp.best.com
Mon Feb 17 23:27:00 EST 1997


Huang Ke-xue (khuang at CHEMVX.CHEM.TAMU.EDU) wrote:
: Hi, Netters,

: I met a serious problem recently. The transformed BL21(DE3)(by a expression
: plasmid) lyses automatically after 6-8 hr growing. I repeated this three times. 
: Do you think my stuff was contamined by Phage(?). 
: Any suggestion?

Try another DE3 lysogen?



More information about the Methods mailing list