E. coli (Bl21DE3) lyses automatically

Huang Ke-xue khuang at CHEMVX.CHEM.TAMU.EDU
Mon Feb 17 19:33:35 EST 1997


Hi, Netters,

I met a serious problem recently. The transformed BL21(DE3)(by a expression
plasmid) lyses automatically after 6-8 hr growing. I repeated this three times. 
Do you think my stuff was contamined by Phage(?). 
Any suggestion?

Thanks


Huang
    




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