DNA recovery by phenol/chloroform extraction or GeneClean

Chris Boyd chrisb at hgu.mrc.ac.uk
Tue Feb 18 06:44:57 EST 1997


Wilson (netson at uxmail.ust.hk) wrote:

(snip)

: I have heard that if DNA with stickly end(s) (digested by restriction
: enzyme e.g. EcoRI) was vortexed, they will stick together and
: consequently inhibit ligation.  This is screw up of DNA,  is it possible
: to occurred?

I don't believe so.  For a start, how could you prove this idea? It's
not sufficient to show that vortexing inhibits ligation, which it could
do if the DNA gets sheared in the process (though shearing problems
only kick in significantly with large (10s of kbs) linear molecules).

Best wishes,
--
Chris Boyd                       | from, | MRC Human Genetics Unit
chrisb at hgu.mrc.ac.uk             |  not  |  Western General Hospital
http://www.hgu.mrc.ac.uk/~chrisb |   for |   Edinburgh EH4 2XU, SCOTLAND



More information about the Methods mailing list