Histone H1 size?
haqr at oci.utoronto.ca
Mon Feb 17 19:01:44 EST 1997
Could anyone tell me the size of phosphorylated Histone H1? I'm using it
for H1 kinase assays with several cdks, and according to Stryer, Histone
H1 is ~20kDa. But I've seen literature where H1 kinase reactions are run
on 10% SDS-PAGE, and if histone H1 runs at ~20kDa, it should be very
near the bottom of the gel!?
On a related note, I see a large number of bands when I run my histone
kinase reactions? Is this common or is something wrong?
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