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E. coli (Bl21DE3) lyses automatically

Justinian jtbrown at ccwf.cc.utexas.edu
Tue Feb 18 12:22:37 EST 1997

On 17 Feb 1997, Huang Ke-xue wrote:

> I met a serious problem recently. The transformed BL21(DE3)(by a expression
> plasmid) lyses automatically after 6-8 hr growing. I repeated this three times. 
> Do you think my stuff was contamined by Phage(?). 
> Any suggestion?

	are you sure it's e.coli?

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