Stop codon read-through?
rafael at howard.genetics.utah.edu
Tue Feb 18 18:08:44 EST 1997
On Mon, 17 Feb 1997, Harris Laboratory wrote:
> Is there any evidence for read-through on a UAG stop codon? If so what
> examples are available?
Yes. In the Moloney Murine Leukemia Virus (MuLV). Check Wills et al (1991),
> We have a cDNA sequence that contains an addition of 45 bp. This
> causes an codon shift that results in a premature stop codon. This
> should yield a 11 kDa protein when translated. However in in vitro
> translation system, a 59 kDa protein is generated & detected via
> Western analysis.
Is there no trace of the predicted 11 KDa protein? Most of the readtrough
cases are not 100%.
> Any help would be appreciated.
We would be very interested in helping you. Taking a look to the
actual sequence could be very helpful; there could be several recoding
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