multiplying bands in gel purification
John G. Luz
jgluz at MAIL.MED.CORNELL.EDU
Tue Feb 18 18:48:29 EST 1997
Folks--I'm tearing my hair out. I cut a nice clean tight band out of a
gel, and purified it with the qiagen gel extraction kit. But when I ran
the gel-purified band out on a gel to check my yield, I got two bands,
both about equimolar. One band looked about the right size. The other
looked about 1kb smaller. The band I gel-purified was 5.4 kb and cut
with NdeI and Xho. What's going on?--John
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