<188.8.131.52.19920216003444.0066b328 at cc.kmc.edu.tw>,
u8251012 at CC.KMC.EDU.TW says...
>Some annoying probrems !!Please give me advices
>drug A induced increased level of TGF-beta protein
>but do not induced increased expression of TGF-beta mRNA
>How can I approach the translational control ??
>It Seems not by nuclear runon/off or mRNA halflife assay ?
TGFbeta protein requires post translational activation by cleavage
by eg. urokinase. Are you selectively looking at the *active*
form of TGFbeta (and ignoring the latent form)? If so your
mystery drug could simply be increasing the activation
rather than having any effects on mRNA at the post-transcriptional
A way to address this would be to assay the latent
and active forms and see if there is actually an increase
in total TGFbeta or simply an interconversion.
I hope that this helps,
Bernard Murray, Ph.D.
bernard at elsie.nci.nih.gov (National Cancer Institute, NIH, Bethesda MD, USA)