expression vector problem

wliu at receptor.pharm.uwa.edu.au wliu at receptor.pharm.uwa.edu.au
Wed Feb 19 01:34:45 EST 1997


Dear netter,
I just cloned my DNA into pKK-223-3 expression vector as a SmaI-PstI
fragment, unfutunately, my DNA is absent of ribosome binding site, and
the start codon in my DNA is 40 base away from the r.b.s of the vector
itself. Can I get any protein expression  as pKK-223-3 is an
over-expression vector? I need my protein expressed in host cell
membrane. It will be appreiated if anybody can give me advice about
this matter.
Williams
Department of Pharmacology
University of Western Australia
Australia





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