expression vector problem

wliu at wliu at
Wed Feb 19 01:34:45 EST 1997

Dear netter,
I just cloned my DNA into pKK-223-3 expression vector as a SmaI-PstI
fragment, unfutunately, my DNA is absent of ribosome binding site, and
the start codon in my DNA is 40 base away from the r.b.s of the vector
itself. Can I get any protein expression  as pKK-223-3 is an
over-expression vector? I need my protein expressed in host cell
membrane. It will be appreiated if anybody can give me advice about
this matter.
Department of Pharmacology
University of Western Australia

More information about the Methods mailing list