Quick transformation protocol wanted

Paul N Hengen pnh at ncifcrf.gov
Tue Feb 18 16:36:59 EST 1997


A.P.N. de Boer (deboer at nefeli.imbb.forth.gr) wrote:

> The fastest I know of is the method of Chung which uses a solution of
> PEG and DMSO made in LB. For very efficient transformation you grow
> cells to mid log, chill on ice, centrifuge and resuspend in 1/10 vol of
> solution. They are ready to use or to freeze. For quick and dirties you
> can make up the solution as a 2X stock and just add an equal volume to
> your cells, no need to centrifuge or anything. It even works with
> overnight cultures, although at reduced efficiencies.

How about resuspending a colony in 1X TSS? Anyone do it this way?
Wasn't there a protocol for resuspending a colony and doing a quick
freeze - thaw, add DNA and plate? I think that's the one you're looking for.

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