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Stable Thp1 transfections

Guriq Basi gbasi at SLIP.NET
Wed Feb 19 21:19:37 EST 1997


hello fellow netters

has anyone out there successfully obtained stable (underlined!!) thp1
cells transfected by any method known to man?  we can obtain 10-20%
transfection efficiency under transient conditions using e.g. ß-gal
reporter driven by cmv promoter by electroporation (biorad gene pulser,
10-20µg DNA per 10 million cells, @ 220/960µF).  however by 72-96 hrs
post transfection, 99.99% of the culture is dead, no need for drug
selection.  if you do add drug, nothing grows out 2-3 weeks post +G418
culture (n=4-6 trials).  has any cracked this nut, or heard of anyone
who has?  all replies will be enthusiastically awaited, and
appreciated.  thank you.

guriq basi



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