multiplying bands in gel purification

Jill Kellogg jkellogg at
Wed Feb 19 18:44:59 EST 1997

John G. Luz wrote:
> Folks--I'm tearing my hair out.  I cut a nice clean tight band out of a
> gel, and purified it with the qiagen gel extraction kit.  But when I ran
> the gel-purified band out on a gel to check my yield, I got two bands,
> both about equimolar.  One band looked about the right size.  The other
> looked about 1kb smaller.  The band I gel-purified was 5.4 kb and cut
> with NdeI and Xho.  What's going on?--John

Just for a lark test the pH of your loading buffer.  You may be denaturing your

J Kellogg
Research Assoc.
Agritope, Inc.
Beaverton OR

All comments/opinions in this message are solely those of the sender, 
and do not necessarily reflect the views of Epitope, Incorporated 
or any of its subsidiaries.

More information about the Methods mailing list