Can anyone tell me how to remove PEG from my DNA solutions?
I have lambda preps that I wish to cut and then spin in a microcon 100 to
separate the oligo linkers, but nothing goes through the filter because of
all PEG, I think (the stock DNA solution is milky white and there is only
5-10 ugs of DNA in the restriction reactions that I am trying to spin.).
Chloroform extraction does not seem to do anything.
Thanks for any tips,
Venkat Pisupati Lab -44-01223-334131
Wellcome/CRC Institute Fax -44-01223-334134
Dept. of Genetics E.mail. vnp at mole.bio.cam.ac.uk
University of Cambridge
UK CB2 1QR