Stop codon read-through?

C. J. Fields cfields at gab.unt.edu
Wed Feb 19 03:03:51 EST 1997


I hate to do this, but I forgot one minor detail about my hypothesis for
this:

Have you tried protein sequencing?  If the mutation is in an area
reachable by Edmon degradation (either directly or from protease-cleaved
frags), then you could get a direct answer.

Also, a suppressor of this type might be very efficient in creating
expression havoc with other genes with UAG stops.  You could check the
sizes of their expressed products to look for increased MW.

Just a thought.......
-- 
C. J. Fields
Graduate Student, Dept. of Biological Sciences
The University of North Texas
Denton, TX 

email : cfields at gab.unt.edu
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