3-Amino triazole conc.?

Martin Gossen martin.gossen at rz.ruhr-uni-bochum.de
Wed Feb 19 16:38:41 EST 1997


Dear colleagues,

does anyone have experience with using 3-Amino triazole for
suppressing background synthesis of Histidine in yeast cells? What
concentration would you recommend in agar plates when conducting a
yeast two hybrid screen with the strain Y190? Did you get positive
protein interactions this way?

Thank you very much for your help.

Martin Gossen
Molecular Human Genetics
Ruhr-University Bochum
Germany




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