refolding from inclusion bodies

bipin k. dalmia dalmiabk at phibred.com
Wed Feb 19 14:52:54 EST 1997


On 19 Feb 1997 08:19:22 -0800, juhi at NCBS.TIFRBNG.RES.IN (juhi juneja)
wrote:

>The mutant protein that I'm trying to purify is expressed in inclusion
>bodies. After solubilising in 8Murea,50%of the protein precipitates
>while dialysing out the urea. Is there any way to prevent precipitation
>and increase refolding yields?

50% refolding yield is pretty good in my experience. i'm usually happy
with 10%.

bip



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