Westerns and estrogen receptors

Aideen O Doherty Aideen.Odoherty at Queens-Belfast.ac.uk
Wed Feb 19 13:04:36 EST 1997


Hi there,
Would anyone know the best way to extract the estrogen receptor from the 
nucleii of tissue cultured cells for running in a western blot?
It is about 67kDa in size (some reports of smaller/larger proteins have been 
noted). I have done this unsuccessfully twice and as the entire process takes 
so long, I want to make sure I am doing all the right things before I 
continue.
Thanks a lot for any suggestions!
Aideen



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