refolding from inclusion bodies

juhi juneja juhi at NCBS.TIFRBNG.RES.IN
Wed Feb 19 11:19:22 EST 1997

The mutant protein that I'm trying to purify is expressed in inclusion
bodies. After solubilising in 8Murea,50%of the protein precipitates
while dialysing out the urea. Is there any way to prevent precipitation
and increase refolding yields?

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