How can I assay translational rate ??

Dr E. Buxbaum EB15 at le.ac.uk
Thu Feb 20 07:46:11 EST 1997


u8251012 at cc.kmc.edu.tw wrote:

>drug A induced increased level of TGF-beta protein
>but do not induced increased expression of TGF-beta mRNA
>
>
>How can I approach the translational control ??
>It Seems not by nuclear runon/off or mRNA halflife assay ?

Measure the incorporation of radioactive amino acids into your protein 
per unit time. Usually done with 35S-methionin or cystein. Incubate with 
the hot amino acid for some time, then some time in cold ones. As you 
vary the times, you can follow the metabolism of your protein. 




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