double labeling with BrDU

Danny Altschuler dla at galactose.mc.duke.edu
Thu Feb 20 10:09:17 EST 1997


Hi,
Does anyone outthere have any protocol for double staining with BrDU that
does not use the HCl denaturing step?
Of course the BrDU staining was OK but the acid step seems to destroy
antigenicity for my protein/antibody (BTW is an HA tagged).
I would appreciate any suggestions.
Thanks 
Danny
Please e-mail me to
altschul at med.unc.edu




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