Pfu polymerase in PCR
duncant at vax.cs.hscsyr.edu
Fri Feb 21 17:51:12 EST 1997
Dr. Duncan Clark wrote:
> In article <330B9556.41C6 at vax.cs.hscsyr.edu>, Tom Duncan
> <duncant at vax.cs.hscsyr.edu> writes
> >My main point was whether any references show that Pfu or other
> >proof-reading polymerases degrade primers in the single-stranded form
> >(that is, primers not annealed to their template).
> Check out Gene Vol 112, pp29-35 (1992) by Wayne Barnes. Bottom right of
> first page.
I checked out the above article, and he only cites his own unpublished
observations and an anonymous note in a newsletter from New England
Biolabs as suggesting cleavage of un-annealed primers. I'm still
doubtful, since excess primers could be depleted as annealed primers are
degraded and more primers have the opportunity to bind and be degraded.
Thomas M. Duncan
Dept. Biochemistry & Molecular Biology
SUNY Health Science Center
750 E Adams St, Syracuse, NY 13210
Email: duncant at vax.cs.hscsyr.edu
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