dam-/dcm- bugs and mutations??

Dr. Duncan Clark duncan at genesys.demon.co.uk
Fri Feb 21 08:41:38 EST 1997

In article <5ejtdt$b7d at scotsman.ed.ac.uk>, Chris Boyd
<chrisb at hgu.mrc.ac.uk> writes
>On the other hand, dam- mutants are defective in strand discrimination
>during mismatch repair and are therefore slightly more mutagenic than
>wild-type.  Not enough to worry about in practice, though, I think.

The sources of information on this newsgroup never ceases top amaze me.
I've used dam and dcm strains for a long time but have totally missed
the above. Your last comment on plasmid yield may also explain why my
JM110 lambda lysogen always gives me 2-3 fold less lambda than my W3110

Many thanks


The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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