SSC conc. in prehyb.

Denni Schnapp ds4 at st-andrews.ac.uk
Fri Feb 21 05:52:40 EST 1997


Hi folks,

We prehybe our filters (in my case it was Hybond-C nitrocellulose) in 6 x SSC,
0.5% SDS, 5X Denhards's (I think that is right) final conc., plus salmon sperm
DNA. Like a moron, I copied the corresponding recipe down wrongly and ended up
with 5 x SSC. Now, some prehyb.-buffers appear to contain 5 x SSC, others contain 6 x SSC. What are peoples experience with the SSC conc.?

Thanks for any input,

Denni (ds4 at st-and.ac.uk)



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