Transposon mutagenesis

Brad Nicholson Brad_Nicholson at hlthsci.med.utah.edu
Sat Feb 22 06:49:51 EST 1997


In article <Pine.SOL.3.91.970221152243.11771A-100000 at howard>, Rafael
Maldonado <rafael at howard.genetics.utah.edu> wrote:

> I disagree. Tn10 has far many more useful variants than Tn5<
>major snip<

Hi Rafa,

I don't think that you can go that far.  See the huge family of mini Tn5
derivatives that Kenneth Timmis has produced.  Reasonable randomness, the
ability to use the system again with another resistance marker, lots of
different markers (Kan, Cam, Tet, Spec, etc.), lac, pho, lux reporters.
Yada, yada, yada.

See de Lorenxo, Herrero, Jakubzik, & Timmis J. Bacteriol. 172(11) p. 6568-6576.

Plus he is willing to send things out.

Brad



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