quick question

Hiranya Roychowdhury hroychow at NMSU.EDU
Sat Feb 22 20:20:42 EST 1997

At 04:04 PM 2/22/97 +0200, Rani Geha wrote:
>What is the difference between transfection and transformation?

These two terms have been used interchangably in literatures. In general, the classical concept of transfection was the introduction of DNA into one cell by another, related or unrelated, cell. However, the term 'transfection' is now used to mean introduction of gene(s) or a piece of DNA into an eukaryoting cell while the other term is used for the same idea with respect to prokaryotic cells. 'Transfection' has  also been used to describe the introduction of viral nucleic acids into a cell by the virus. 

'Transformation' may also mean the transition to immortality by a cell(as in neoplastic cells). 

Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

More information about the Methods mailing list