Sequencing and sharkteeth

[martin LEACH]

I use a couple of bulldozg clips...clamp them to the top of the gel after
inserting comb....3-4 spanning the whole gel...

you should see the crusty acrylamid that builds up after many years....

that takes skill!

M

Randy Willis (willis at gandalf.psf.sickkids.on.ca) wrote:
: Jørgen Stenvik wrote:
: > 
: > We have some trouble with leakage using sharkteeth combs for sequencing.


: Jorgen,

: We used to use rubber-insulated clamps in the middle of the glass plates
: to hold them together more tightly.  You can probably find them in a
: hardware store or anywhere that sells electrical supplies.  Once your
: sample has run into the gel, you can remove the clamps safely.

: Good luck

: Randall C Willis, Researcher
: Biochemistry, Hosp for Sick Children
: 3522-555 University Ave
: Toronto, ON
: M5G 1X8  CANADA

: 416-813-5933 (ph)  -5022(fax)

: willis at gandalf.psf.sickkids.on.ca

--
.....          Martin Leach                Email:leach at bu.edu
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323
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