Sequencing and sharkteeth
leach at bu.edu
Sun Feb 23 23:16:35 EST 1997
I use a couple of bulldozg clips...clamp them to the top of the gel after
inserting comb....3-4 spanning the whole gel...
you should see the crusty acrylamid that builds up after many years....
that takes skill!
Randy Willis (willis at gandalf.psf.sickkids.on.ca) wrote:
: Jørgen Stenvik wrote:
: > We have some trouble with leakage using sharkteeth combs for sequencing.
: We used to use rubber-insulated clamps in the middle of the glass plates
: to hold them together more tightly. You can probably find them in a
: hardware store or anywhere that sells electrical supplies. Once your
: sample has run into the gel, you can remove the clamps safely.
: Good luck
: Randall C Willis, Researcher
: Biochemistry, Hosp for Sick Children
: 3522-555 University Ave
: Toronto, ON
: M5G 1X8 CANADA
: 416-813-5933 (ph) -5022(fax)
: willis at gandalf.psf.sickkids.on.ca
..... Martin Leach Email:leach at bu.edu
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