Na+/K+ ATPase assay

lincoln at indigo.ie lincoln at indigo.ie
Sun Feb 23 12:13:33 EST 1997


In article <5elbh8$ho0 at pulp.ucs.ualberta.ca>, jhance at gpu5.srv.ualberta.ca
(J Hance) wrote:

> Hi, I need to measure Na+/K+ ATPase in cultured cells, but only have an
> assay which can be used in prepared vesicles. If anyone out there has
> such a protocol I would be interested to hear about it. Thanks.
> 
> Jackie Hance(grad student)
> Department of Biochemistry
> University of Alberta

Hi Jackie,

One possible approach to measuring the activity of the Na+/K+-ATPase is to
use its ability to substitute 86Rb+ for K+.
Any paper dealing with the measurement of neuronal/synaptosomal plasma
membrane potentials should be helpful.
Good luck,

lincoln



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