Na+/K+ ATPase assay

[lincoln at indigo.ie]

In article <5elbh8$ho0 at pulp.ucs.ualberta.ca>, jhance at gpu5.srv.ualberta.ca
(J Hance) wrote:

> Hi, I need to measure Na+/K+ ATPase in cultured cells, but only have an
> assay which can be used in prepared vesicles. If anyone out there has
> such a protocol I would be interested to hear about it. Thanks.
> 
> Jackie Hance(grad student)
> Department of Biochemistry
> University of Alberta

Hi Jackie,

One possible approach to measuring the activity of the Na+/K+-ATPase is to
use its ability to substitute 86Rb+ for K+.
Any paper dealing with the measurement of neuronal/synaptosomal plasma
membrane potentials should be helpful.
Good luck,

lincoln