Cutting DNA from non-E.coli

Eileen Hickey hickey at cmb.UNR.EDU
Mon Feb 24 17:21:14 EST 1997


We're trying to do Southern blots to test a probe for cloning genes from 
several bacteria: Legionella, Neisseria meningitidis, S. pneumoniae, and S. 
pyogenes. The DNAs we have are not cutting well, and it occurs to us that they 
may be modified? Has anyone cloned any genes from these bacteria? and what did 
you cut with?



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