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quick question

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Mon Feb 24 10:58:02 EST 1997

In article <5er8nv$3lo at light.nih.gov>, bernard at elsie.nci.nih.gov (Bernard
Murray) wrote:

>Transfection: Transient transfer of DNA to host
>(plasmid into mammalian cells that is expressed but not selected)

Hmmm...what about stable *transfectants* using a selectable marker like
neo, puro, hygro or zeo?

>Transformation: Stable transfer of DNA to host
>(so plasmid into bacteria, episomal or integrated plasmid in
>eukaryotic cells)

...and some oncogenesis-studying friends would take the tack that
transformation is immortalization, loss of contact inhibition, ability to
grow in soft agar, reduced serum requirement, etc..

The underlying commonality of the answers is - getting exogenous DNA into
a cell.

>         Now if you want to get a real argument going around here
> try "the difference between run-on and run-off transcription...".

AARRRGGGHHH...not that....anything but that ;-)
<then again it could be worse...remember the "sense vs antisense" debate ;-)


Karl the hepB guy

Karl Fischer
tyr-2 at bones.biochem.ualberta.ca

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