In article <5er8nv$3lo at light.nih.gov>, bernard at elsie.nci.nih.gov (Bernard
>Transfection: Transient transfer of DNA to host
>(plasmid into mammalian cells that is expressed but not selected)
Hmmm...what about stable *transfectants* using a selectable marker like
neo, puro, hygro or zeo?
>Transformation: Stable transfer of DNA to host
>(so plasmid into bacteria, episomal or integrated plasmid in
...and some oncogenesis-studying friends would take the tack that
transformation is immortalization, loss of contact inhibition, ability to
grow in soft agar, reduced serum requirement, etc..
The underlying commonality of the answers is - getting exogenous DNA into
> Now if you want to get a real argument going around here
> try "the difference between run-on and run-off transcription...".
AARRRGGGHHH...not that....anything but that ;-)
<then again it could be worse...remember the "sense vs antisense" debate ;-)
Karl the hepB guy
tyr-2 at bones.biochem.ualberta.ca