In our lab we study the dynamics of microbial communities in soil. One
of the techniques we use for this is DGGE/TGGE of 16S rRNA gene
fragments, amplified with GC-clamped PCR primers. In literature there
are some references to DGGE/TGGE in which 5'-psoralen-labelled primers
are used in stead of the usual GC-clamped ones. After amplification the
two DNA strands can be covalently linked by UV-irradiation.
Psoralen-labelled primes are available from various suppliers, but are
rather expensive. That's why I would like to know if there are
researchers out there who have experience with the use of these primers
for DGGE/TGGE. Any comment on this is welcome.
Natl. Inst. of Publ. Health and the Environment, Bilthoven, the