help with pcr for dna from sediments
Guenter.Jost at io-warnemuende.de
Mon Feb 24 04:24:56 EST 1997
we extracted dna from different sediments (dark ones as well as sandy)
but had no success in amplification of 16s rdna with eubacterial primers.
Also different extraction protocols brought no success. When we add
bacteria to the sediment we also failed with pcr. As next step we tried
dilution of dna - the same negative result.
Now we are looking for the easiest, simplest and cheapest way out.
I forgot to mention that we also tried cleaning dna with
gelelectrophoresis (with and without pvpp) because hoping that humic
substances will bind to it.
Baltic Sea research institute
guenter.jost at io-warnemuende.de
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