3-Amino triazole conc.?

Andy amcshea at world.std.com
Mon Feb 24 20:49:09 EST 1997

Martin Gossen (martin.gossen at rz.ruhr-uni-bochum.de) wrote:
: Dear colleagues,

: does anyone have experience with using 3-Amino triazole for
: suppressing background synthesis of Histidine in yeast cells? What
: concentration would you recommend in agar plates when conducting a
: yeast two hybrid screen with the strain Y190? Did you get positive
: protein interactions this way?

5mM, yes.

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