Q:measurement of protein's surface hydrophobicity

Kurt L Brillhart klbrillhart at ccgate.dp.beckman.com
Sun Feb 23 10:04:56 EST 1997


The simplest way to do this might be to simply measure the degree of retention 
on a hydrophobic (not reverse phase) support such as phenyl and/or octyl 
sepharose. One would reasonably expect that the support would have a high 
binding capacity for proteins with high surface hydrophobicity, particularly 
in the presence of molar concentrations of ammonium sulfate.  The material is 
readily available from Pharmacia and can easily be packed into small columns 
that can be cycled very quickly.

Kurt Brillhart
klbrillhart at ccgate.dp.beckman.com



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