Protein-protein interaction of His-tag of pET14b?

Jimmy S.H. Tsang jshtsang at hkucc.hku.hk
Wed Feb 26 01:47:31 EST 1997


Dear All,
        I have recently fused a bacterial gene downstream of the His-tag 
sequence of the pET14b from AMS Biotech.  The in vitro transcribed and 
translated protein forms a complex which migrates much slower than the wild 
type protein in a native PAGE.  Did anyone experience a similar 
'multimerisation' problem?  The same gene cloned into pET19b produced a 
protein similar to that of native protein, i.e. no 'multimerisation'.  Any 
suggestion and comment will be appreciated.

J.S.H. Tsang
Department of Botany
The University of Hong Kong
jshtsang at hkucc.hku.hk
http://www.hku.hk/botany/staff/tsang.html



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