WANTED: Vector to express MF-alpha fusion protein
JIrving at its-med.cc.monash.edu.au
Wed Feb 26 15:35:19 EST 1997
I was wondering if anyone out there has a vector containing the prepro
sequence of mating factor-alpha to direct the secretion of a recombinant
protein in S. cerevisiae. I need one that has a cloning site C-terminal
to (at the 3' end of) the KEX2 protease cleavage site of the prepro
sequence; and also containing a suitable promoter allowing regulated
expression of the MFalpha-fusion recombinant protein.
I would greatly appreciate any help/advice that you may have!
Department of Biochemistry and Molecular Biology
More information about the Methods