Northern blot RNA degradation

Jos Jos.Broers at molcelb.rulimburg.nl
Wed Feb 26 13:30:30 EST 1997


Dear Reader,

I am performing Northern blot hybridizations and wonder how sensitive
RNA is for degradation after cross-linking to a Nylon (or
nitrocellulose) membrane. Will this degradation interfere with
hybridization efficiency? I am asking this since I use BSA in my
hybridization solution which might contain RNase. Can I get rid of RNase
in BSA?
Many thanks for your response
Jos



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