Transient transfection of Cos-7 (electroporation v DEAE dextran)
STEEVE at bio1.lan.mcgill.ca
Mon Feb 24 13:37:52 EST 1997
In article <3309DBA6.7829 at sheffield.ac.uk>, K.Mulcahy at sheffield.ac.uk wrote:
> does anyone have any experience with transient transfection of Cos-7
> cells? I have attempted a DEAE-dextran/chloroquine based procedure but
> only succeeded in transfecting 20-30% of the cells with a ß-gal reporter
> plasmid (tested 24 hours post transfection). Does anybody else succeed
> in obtaining efficiencies much higher than this (say 50-70%)?
> Also, does anyone have any experience of using electroporation for the
> transient transfection of Cos-7 cells? If so, could you please let me
> know what electroporation conditions you use and what transfection
> efficiencies you are able to obtain.
> Many thanks,
> Kevin Mulcahy
Look for something called polybrene (SIGMA). This compound, combined with
DMSO give high yield... can't tell no more. You'll have to look for the
protocol in litterature.
Hope this helps.
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