Clontech GFP

Alex Harvey harvey at bscr.uga.edu
Wed Feb 26 14:07:44 EST 1997


Is anyone using Clontech's pEGFP reporter vectors?  I want to compare
expression of the F64L/S65T double mutant to the same with "humanized"
codons.  Clontech claims the change in codon usage results in around a
7-fold increase in fluorescent signal (in CHO cells) - can anyone verify
this (in any cell type)?  I'm using GFP as a reporter in chicken
blastodermal cells.

Thanks for any input.

Gord Speksnijder
Department of Genetics
B410 Life Sciences
University of Georgia
Athens, GA 30602-7229
phone (706) 542-1420
fax   (706) 542-1413
email  gord at bscr.uga.edu



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