blotting acrylamide gels

John Watson watson_j at bms.com
Wed Feb 26 14:05:39 EST 1997


Constable Julie Lynn wrote:
> 
> Does anyone have a protocol for blotting DNA from an acrylamide gel onto a
> nylon membrane?  I seem to recall that it is a fairly simple procedure,
> like putting a moistened membrane on top of the gel with some weight on
> top.  Any ideas would be greatly appreciated.

Hmmm, I don't recall *anything* about capillary transfers out of 
polyacrylamide.  I've always electroblotted.  I don't remember the exact 
transfer conditions but I'm pretty sure I can dig them out if need be.

----------------
John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
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