does anyone have a spac-promoter vector

H. Yim hyim at u.washington.edu
Thu Feb 27 12:02:27 EST 1997


I think the original reference is:  Henner, D.J.  1990.  Methods in Enz.
185:223-228.  

Harry Yim Ph.D.
Mailstop:CH-32
Dept of Infectious Diseases
Children's Hospital and Medical Center
Seattle WA 98105

hyim at chmc.org  
hyim at u.washington.edu
(206) 528-2767
fax (206) 527-3890


On Wed, 26 Feb 1997, Neil Mattatall wrote:

> Hi,
> 
> I have been trying to get a hold of a plasmid that contains the
> spac-promoter. spac is a promoter hybrid that contains the lac operator
> region, and is used to modulate expression through IPTG induction in B.
> subtilis.  The original vector came with the constitutively expressed
> lacI gene.   Does anyone have this vector???
> 
> Thanks for your help,
> 
> All the best,
> 
> Neil
> 
> -----------------
> Neil R. Mattatall                       Phone: (613) 545-6000 (ext.
> 5189)
> Queen's University                      Fax:   (613) 545-2497
> Department of Biochemistry              Email: 6nrm at qlink.queensu.ca
> Kingston, Ontario K7L 3N6
> Canada
> 
> 




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