IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Making Nickel resin??

wegloehner at mpimg-berlin-dahlem.mpg.de wegloehner at mpimg-berlin-dahlem.mpg.de
Thu Feb 27 12:43:15 EST 1997

In article <Pine.PMDF.3.95.970227091705.539244773A-100000 at rubens.its.unimelb.edu.au>, Stephen W Doughty <swd at rubens.its.unimelb.edu.au> writes:
> Hi, 
> I interested in making my own metal affinity resin to purify a (HIS)6
> tagged protein, can anyone tell me where I can get a protocol from to
> do this?
> thanks 
> steve
> Stephen Doughty ( swd at rubens.unimelb.edu.au )/Churchill's commentary on man:
> Centre for Animal Biotechnology             /Man will occasionally stumble over
> School of Veterinary Science               /the truth, but most of the time he
> University of Melbourne, VIC, Australia   /will pick himself up & continue on.

dear stephen,

you may have a look into the following paper.

New metal chelate adsorbent selective for proteins and peptides
containing neighbouring histidine residues. Hochuli et al. (1987)
Journal of Chromatography, 411, 177-184

describes the preparation of Ni-NTA-sepharose from chemicals everyone can buy
this paper is also the basis of the Ni-NTA patent hold by Hoffmann-La Roche
licence by QIAGEN

there are also several references in this paper about the preparation of 
Iminodiaceticacid IDA chelating material.

hope this helps


More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net