In article <Pine.PMDF.3.95.970227091705.539244773A-100000 at rubens.its.unimelb.edu.au>, Stephen W Doughty <swd at rubens.its.unimelb.edu.au> writes:
> I interested in making my own metal affinity resin to purify a (HIS)6
> tagged protein, can anyone tell me where I can get a protocol from to
> do this?
>> Stephen Doughty ( swd at rubens.unimelb.edu.au )/Churchill's commentary on man:
> Centre for Animal Biotechnology /Man will occasionally stumble over
> School of Veterinary Science /the truth, but most of the time he
> University of Melbourne, VIC, Australia /will pick himself up & continue on.
you may have a look into the following paper.
New metal chelate adsorbent selective for proteins and peptides
containing neighbouring histidine residues. Hochuli et al. (1987)
Journal of Chromatography, 411, 177-184
describes the preparation of Ni-NTA-sepharose from chemicals everyone can buy
this paper is also the basis of the Ni-NTA patent hold by Hoffmann-La Roche
licence by QIAGEN
there are also several references in this paper about the preparation of
Iminodiaceticacid IDA chelating material.
hope this helps