Hot plasmid + Cold Vector = Insert-specific probe????

Michael J. Prigge prigge at darkwing.uoregon.edu
Thu Feb 27 18:46:45 EST 1997


I need to screen a cDNA library with a BAC with a ~100 kb insert. 
I thought I read somewhere that one could label the whole plasmid, 
then add lots of cold vector to the hyb solution. This might 
theoretically result in no hybridization to the vector sequences 
of the plaques. 

Has anybody done this? Any replies would be appreciated.
Mike
Mike Prigge                    prigge at darkwing.uoregon.edu
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