Hirt procedure pl.

[Patil J G]

Dear Netters,
We wish to employ Hirt procedure to isolate plasmid DNA from the genomic 
DNA of cultured cells. We have read the original paper of Hirt 1967. I am 
sure lot more of you will have great tips and may be modifications that 
would be of great help. We shall greatly appriciate your comments and 
suggestions. I am sure your experinces will help us avoid pit-falls. 
Please try and be as explanatory as possible. Thanks in advance.
Jawahar.

--
**********************************
Jawahar G Patil                  :
Department of Biochemistry       :
National University of Singapore :
Singapore-119260                 :
E-mail: bchjgp at nus.sg            : 
Phone: (65)-7727996(O)           :
       (65)-7742867(R)           :
Fax:   (65)-7791453              :
**********************************