Dexamethasone-inducible pMAMneo vector

Marit Anthonsen Marit.Anthonsen at medkem.lu.se
Fri Feb 28 10:44:28 EST 1997


I have performed stable transfections in CHO-cells using the pMAMneo
mammalian expression vector from Clontech. The gene expression should be
dexamethasone-inducible. Unfortunately, we have problems. Tthat is: the
induction has worked ONCE (for 37 clones) - 4 unsuccesful tries. I am
wondering if anyone has experience with this vector, and if there is a
"trick" (eg. in treatment of the dexamethasone?)

Marit.



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